High Throughput HPLC with a Minimum
of Hardware
- Single-Point Control of Two Independent Gradient Streams
- Dual Column Processing
- Staggered Injections From Alternating Sample Sets
- Effectively Doubled Sample Throughput
- Off-line Column Conditioning
- Stream Switching Valves With Flexibility For The Application
The SIL-HT Dual Gradient System (DGS) will improve sample throughput
in your laboratory with a minimum of hardware. The built-in system
controller enables the DGS with several advanced pumping modes.
Dual Binary Gradient mode delivers single point control of two
independent gradient LC streams for dual column processing and
staggered injections. As an LC front end for mass spectrometry,
the DGS simply makes sense, delivering spot-on repeatability and
greatly improved sample throughput, alleviating the chromatography
bottleneck typical of a single column approach.
The system uses two integral 6-port, stream selection valves to
direct flow on-demand into the injection path, then into the analysis
column and detector of choice. In a typical plumbing scheme, one
valve directs the LC stream into the injector path, and the other
valve directs the analysis stream to the detector of choice or
to waste. The utmost in flexibility according to the application
is realized with the use of time program events for stream selection.
The DGS can significantly increase overall sample throughput by
eliminating time lost to column conditioning, and a column stream
may be directed to the detector(s) of choice at the user's discretion.
In laboratories such as drug metabolism and pharmacokinetics (DMPK),
throughput is critical and time cannot be wasted waiting for systems
to equilibrate. Using the DGS, two general approaches are used
to automate this process; Alternating Analysis Files or a Single
Analysis File with Pretreatment. Either way, throughput can be
doubled with even the most complex gradient profiles and column
conditioning needs.
In such environments, the dual column, dual sample set approach
also ensures data integrity by injecting all samples from a specific
sample set onto one specific column. In practice, the DGS draws
samples in an alternating fashion from opposing sample racks for
injection into alternating column streams.
The SIL-HT DGS is built around the strengths of Shimadzu's highly
successful SIL-THC autosampler, offering high speed (15s), excellent
repeatability and near zero sample carryover from its needle-in-the-flow-path
design and a patent-pending needle surface processing technology.
Even with the additional stream selection valves of the DGS, the
SIL-HT can inject highly basic and absorbent samples with undetectable
carryover levels. The autosampler provides control of sample temperatures
from 4 to 40ºC.
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Front Ends for Mass Spectrometry
