Accuspot , a new MALDI plate spotter,
automates LC micro-fractionation, spotting and preparation for
MS Analysis. With the AccuSpot system, LC eluent can be accurately
and continuously spotted onto target plates in volume in 10nl or
more on up to nine 96 or 384 format or 18 2x2 format target plates.
By using in tandem with any HPLC system, including the 2D Micro-HPLC
system, proteome analysis can be performed with a higher degree
of precision and with a greater sequence coverage.
LC/MS/MS is often regarded as the technique of
choice in identifying components from complex mixtures; however,
it is not without its limitations. An alternative approach is to
consider LC/MALDI.
LC/MALDI has a number of advantages in that the LC is decoupled
from the MALDI mass spectrometer. MALDI can support:
- A higher data acquisition at the top of the eluting peak (owing
to the fact that the peak can be spread over several spots) allows
a precise selection of precursor ions for MS/MS analysis
- Sample archiving (the MALDI plate can be stored and used to further
validate protein identification if required)
- Uncompromised sensitivity (LC/MALDI analysis can focus solely
on the peak apex) Increased depth of coverage of the sample and
enhanced sequence coverage for the detected proteins.
The AccuSpot was designed for online collection
of fractions eluting from Capillary/Nano LC.
- Supports micro-fractionation on Shimadzu MALDI-TOF/MS targets,
for 96 or 384 well, and ABI format MALDI plates.
- Multiple plates can be spotted continuously. The plate changer
allows a maximum of 9 Shimadzu (MTP format) or 18 ABI plates to
be spotted without stopping.
- Matrix solution is added automatically. Depending on the application,
matrix solution can be mixed with the column eluent via a "T" prior
to the probe or at the probe tip through a 3 duct system. The third
duct is used for rinsing the probe tip.
- Easy to use software gives the user total control over the spotting
operation from setting matrix flow rate to spotting pattern to
report generation.
- The system includes a CCD camera as a standard accessory, so
the actual spotting activity and status can be confirmed on the
PC screen.
- An optional barcode reader is available for sample tracking.
- Compatible with any HPLC system capable of delivering micro/capillary/nanoflow.
The AccuSpot automates LC micro-fractionation,
spotting and preparation for MS analysis. With the AccuSpot system,
LC eluent can be accurately and continuously spotted onto target
plates, in trace amounts (eg.50nl). This allows for automatic preparation
of target plates for MALDI-TOF-MS measurements. When used in conjunction
with Shimadzu's Two-Dimensional Micro LC System and Shimadzu
Biotech's AXIMA MALDI-TOF instruments, proteome analysis can
be performed with a higher degree of precision and with greater
sequence coverage.
Features
Unique sample spotting mode
The "Half-Contact" spotting method (see above) uses
plate sensors (patent pending) to ensure accurate spotting and
virtually eliminates carryover from spot to spot.
Click
here to see the AccuSpot in action.
High sample capacity
9 Shimadzu or 18 ABI target plates can be loaded at one time.
Highly flexible sample loadings
The matrix solution can be added at flow rates from 0.1µL/min
to 50 µL/min. To accommodate different needs the spotting
direction can be either straight row and column or zigzag row and
column.
Monitoring Capability
To ensure that the droplet formation and collection is consistent,
a CCD camera is used to monitor micro-fractionation.
Spotting Modes
Spotting is carried out in one of two ways: Continuous Mode to
collect the entire chromatographic run or Programmable Time Window
Mode to collect only the areas of interest in the chromatographic
run. Both modes allow spotting intervals from 5 to 60 sec/spot.
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Proteomics LC
