LCMS Front End

Shimadzu Front End systems provide integrated solutions to the challenges facing analysts using quadrupole (single or triple quad) or ion-trap MS instruments. The combination of a binary high-pressure LC10ADvp or LC10ADvpm pumping system with DGU14A/M degasser coupled to a SIL-HT (ambient or cooled) autosampler and optional CTO10ASvp column oven is rapidly becoming the new industry standard delivery system for MS users. In an independant study by Covance laboratories in the US, the SIL-HT was shown to provide the best carryover, coupled with fast cycle times with no rinsing, out of the top five "low carryover" systems.

- Features High-Speed, Low Carryover Autosamplers
- Pulse-Free Solvent Delivery from 1uL/min to 150 mL/min
- Multiple Gradient Control Options
- 2-D Options for Automated On-Line Proteomics R&D
- Control embedded in Analyst®, MassLynx®, and Xcalibur® Software Platforms

Totally flexible and reliable, Shimadzu is your one source for LCMS Front Ends. With modular components that allow custom configurations for any Mass Spectrometry environment, Shimadzu delivers the high-performance system you need.

SIL-HT Dual Gradient System

High Throughput HPLC with a Minimum of Hardware

- Single-Point Control of Two Independent Gradient Streams
- Dual Column Processing
- Staggered Injections From Alternating Sample Sets
- Effectively Doubled Sample Throughput
- Off-line Column Conditioning
- Stream Switching Valves With Flexibility For The Application

The SIL-HT Dual Gradient System (DGS) will improve sample throughput in your laboratory with a minimum of hardware. The built-in system controller enables the DGS with several advanced pumping modes. Dual Binary Gradient mode delivers single point control of two independent gradient LC streams for dual column processing and staggered injections. As an LC front end for mass spectrometry, the DGS simply makes sense, delivering spot-on repeatability and greatly improved sample throughput, alleviating the chromatography bottleneck typical of a single column approach.

The system uses two integral 6-port, stream selection valves to direct flow on-demand into the injection path, then into the analysis column and detector of choice. In a typical plumbing scheme, one valve directs the LC stream into the injector path, and the other valve directs the analysis stream to the detector of choice or to waste. The utmost in flexibility according to the application is realized with the use of time program events for stream selection.

The DGS can significantly increase overall sample throughput by eliminating time lost to column conditioning, and a column stream may be directed to the detector(s) of choice at the user's discretion. In laboratories such as drug metabolism and pharmacokinetics (DMPK), throughput is critical and time cannot be wasted waiting for systems to equilibrate. Using the DGS, two general approaches are used to automate this process; Alternating Analysis Files or a Single Analysis File with Pretreatment. Either way, throughput can be doubled with even the most complex gradient profiles and column conditioning needs.

In such environments, the dual column, dual sample set approach also ensures data integrity by injecting all samples from a specific sample set onto one specific column. In practice, the DGS draws samples in an alternating fashion from opposing sample racks for injection into alternating column streams.

The SIL-HT DGS is built around the strengths of Shimadzu's highly successful SIL-THC autosampler, offering high speed (15s), excellent repeatability and near zero sample carryover from its needle-in-the-flow-path design and a patent-pending needle surface processing technology. Even with the additional stream selection valves of the DGS, the SIL-HT can inject highly basic and absorbent samples with undetectable carryover levels. The autosampler provides control of sample temperatures from 4 to 40ºC.

Two-Dimensional HPLC Systems

Meeting & Exceeding Requirements

The high performance 2D micro-HPLC system can perform seperation of complex peptide mixtures with a level of sensitivity and resolution that meets or exceeds present-day requirements.

This high-performance 2D capillary-to-microflow HPLC system co-developed by Shimadzu and the NIH (National Institutes of Health) performs seperation of complex peptide mixtures with a level of sensitivity and resolution that complements present 2D gel electrophoresis.

Features

It is possible to analyze proteins that cannot be analyzed with 2D gel electrophoresis
Highly iconic proteins and hydrophobic proteins can be analyzed on-line.

Automatic analysis with high resolution and sensitivity is possible for proteins with low expression levels
Protein digests can be analyzed automatically by simply placing them into an autosampler. Assay sensitivity enables trace peptide analysis not always detected on 2-D gels.

Stable analysis is ensured by on-line sample trapping and automatic trapping and automatic desalination followed by RPHPLC
A sample from the cation exchange column is trapped on 6 mini trap columns (polymer packed reversed-phase columns with a rentention power equivalent to C4) for desalination, followed by reversed-phase analysis. This method ensures stable, precise analysis.

Multiplex HPLC Systems

Multi-channel HPLC provides an economical solution to high-speed, high throughput analysis due to the need for less equipment. The Shimadzu multiplex system offers parallel gradient analyses on up to 4 columns. The system utilizes one controller and 8 pumps for the ultimate in retention time reproducibility - no more worries about differential column back pressure affecting flow rate accuracy on a "one pump split flow" system.

Coupled with the load autosampler, equipped with a 4-channel injection valve, the Shimadzu multiplexed front end is the ultimate robust, reliable, and reproducible solution to your parallel analysis needs.

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